The FBI's special agent in charge of the Miami Field Office, Jeffrey Veltri, says the agency views the investigation as "extremely serious and are determined to provide answers".
He said the agency was working to pursue and execute a number of search warrants
So far these warrants include a video recording device, the subject's cellular device, a vehicle, and other electronic devices located at previous known addresses.
Mr Veltri said the FBI was working with the Secret Service to complete "numerous interviews" of agents on scene.
Seven civilian interviews have already been conducted. Ryan Wesley Routh's friends, family members, and colleagues will also be questioned.