National Australia Bank will slash more than 400 jobs, adding to the number of workers losing employment in the sector, after ANZ's mass redundancy announcement on Tuesday.
NAB, Australia's second largest bank, will make the cuts in its technology and enterprise operations division, a move it says will simplify team structures.
"Like many businesses, we regularly look at the way we work and how we're structured to deliver the best experience we can for our customers," a NAB spokesperson said.
"While some roles are no longer required or may move location, we are also creating new roles across all locations as necessary, to ensure we are set up for success and can deliver better outcomes."
The Finance Sector Union criticised the lender's decision to cut the roles at the same time as hiring for more than 120 new positions in India and Vietnam, shifting local work overseas.
The FSU said more than 700 workers will be impacted by the changes, which will see permanent cuts to 410 jobs.
The NAB spokesperson said having a global workforce would help the business to "extend hours of operation, speeding up processes and improving turnaround times for customers".
NAB's move to slash its workforce has come just a day after its rival ANZ revealed its plan to terminate 3,500 workers by next September.
The FSU, which is representing affected workers, said NAB's job cuts would shift work performed in Australia overseas.
"Two banks in two days slashing jobs, it's shameful," the FSU's national president Wendy Streets said.
"This isn't one rogue bank, it's the whole sector driving the same agenda at the expense of workers and communities."
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